Rapid separation of bacteriorhodopsin using a laminar-flow extraction system in a microfluidic device     

Yun Suk Huh  Chang-Moon Jeong  Ho Nam Chang  Sang Yup Lee  Won Hi Hong    Tae Jung Park 《Biomicrofluidics》2010,4(1)

A protein separation technology using the microfluidic device was developed for the more rapid and effective analysis of target protein. This microfluidic separation system was carried out using the aqueous two-phase system (ATPS) and the ionic liquid two-phase system (ILTPS) for purification method of the protein sample, and the three-flow desalting system was used for the removal of salts from the sucrose-rich sample. Partitioning of the protein sample was observed in ATPS or ILTPS with the various pHs. The microdialysis system was applied to remove small molecules, such as sucrose and salts in the microfluidic channel with the different flow rates of buffer phase. A complex purification method, which combines microdialysis and ATPS or ILTPS, was carried out for the effective purification of bacteriorhodopsin (BR) from the purple membrane of Halobacterium salinarium, which was then analyzed by sodium dodecyl sulfatepolyacrylamide gel electrophoresis and matrix-assisted laser desorption∕ionization time-of-flight. Furthermore, we were able to make a stable three-phase flow controlling the flow rate in the microfluidic channel. Our complex purification methods were successful in purifying and recovering the BR to its required value.  相似文献   

A study of Korean middle school students’ interests in biology and their implications for biology education     

Jung‐Lim Hong  Kew‐Cheol Shim  Nam‐Kee Chang 《International Journal of Science Education》2013,35(8):989-999

This study analyses middle school students’ interests in biology to provide information on instructional strategies and curriculum development in Korea. An instrument that measures interests was developed based on the middle school biology curriculum. It consists of 64 three‐point Likert‐type items (scores range from 0 to 2). The two components, ‘topic’ and ‘activity’, are composed of 64 items. The topic component and the activity component contain 11 domains and six domains respectively. Data were collected from 267 middle school students in Seoul (male: 169, female: 107). The levels of interest in topic and activity were almost the same and not high. The highest interest domains were ‘genetics’ in topics and ‘observing through a microscope’ in activities. The interest levels in the domain ‘plant morphology’ and ‘plant‐related activity’ were the lowest in each component. The interest level of the eighth graders was the highest found among students. Interest levels of females were lower than those of males in ‘environment and ecosystem’, ‘animal reproduction’ and ‘animal morphology’, but higher in ‘plant morphology’ and ‘plant related activity’ (p < 0.05). Among the topics, the ‘human biology’ domain correlated positively with the domains ‘genetics’, ‘environment and ecosystem’ and ‘respiration of animal and plant’. The correlation between the domains of ‘plant reproduction and nutrition’ and ‘plant development’ was significant in ‘activity’ (r > 0.50, p < 0.001). Consistencies in interests were suggested with respect to instructional strategy and curriculum design.  相似文献   

Exploring regional and institutional factors of international students' dropout: The South Korea case          下载免费PDF全文

Jisun Jung  Yangson Kim 《Higher Education Quarterly》2018,72(2):141-159

With an increasing number of international students in South Korea, the issue of retaining these students and reducing their dropout rates has become important. This study explores the multilevel factors that affect the institutional dropout rate among international students in Korea. The research questions are as follows: (a) to what extent do international students drop out from undergraduate or short‐term programmes in Korean universities?; (b) what are the regional and institutional factors that determine international students' dropout rates? This study uses the panel data on educational institutions as found in the Higher Education in Korea report, which has been updated annually by the Ministry of Education since 2013. According to the pooled ordinary least square and panel analyses, the results indicate that a range of institutional factors (such as type, size, tuition and research performance of academics) and regional factors (such as regional gross domestic product, inflation and the numbers of foreigners in the region) are all associated with the dropout rates of international students. This study suggests that we need to consider various factors to improve the international students' learning experiences both inside and outside of the campus.  相似文献   

The Evolution of Leisure and Sport Participation in Poland     

Bohdan Jung 《国际体育史杂志》2013,30(5):727-741

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Prelinguistic vocal development in young cochlear implant recipients and typically developing infants: year 1 of robust hearing experience     

Ertmer DJ  Jung J 《Journal of deaf studies and deaf education》2012,17(1):116-132

This investigation examined the time course and sequence of prelinguistic vocal development during the first year of cochlear implant (CI) experience. Thirteen children who were implanted between 8 and 35 months and 11 typically developing (TD) infants participated in this longitudinal study. Adult-child play interactions were video- and audio-recorded at trimonthly intervals for each group, and child utterances were classified into categories representing progressively more mature productions: Precanonical Vocalizations, Basic Canonical Syllables, and Advanced Form vocalizations. Young CI recipients met the 20% criterion for establishment of the Basic Canonical Syllables and Advanced Forms levels with fewer months of robust hearing experience than the TD infants. Most CI recipients followed the sequence of development predicted by the Stark Assessment of Early Vocal Development-Revised. The relatively rapid progress of the CI children suggests that an earlier period of auditory deprivation did not have negative consequences for prelinguistic vocal development. It also supports the notion that young CI recipients comparatively advanced maturity facilitated expeditious auditory-guided speech development.  相似文献   

Philosophy of Science and Education     

Walter Jung 《Science & Education》2012,21(8):1055-1083

This is a vast and vague topic. In order to do justice to it one has to write a book or maybe more than one. For it can be understood in quite different ways and on different levels For example you may think mainly of the historical aspect, that is how philosophy of science developed in the last hundred or so years and how its influence on education changed; you may think of quite different schools of philosophy, from Marxist or positivist to such exotic but at some places influential philosophic positions like that of Rudolph Steiner; of course, you may limit the subject to special fields like epistemology, theory of scientific methodology, or, what has become fashionable recently, sociology of knowledge which may have a considerable bearing on physics teaching (Collins and Shapin 1983; Jung 1985). Again we may think of the topic treated by a philosopher, a scientist, an educationalist, a teacher, which would mean quite a difference. I am trying here to speak as an educationalist, with the physics teacher in mind: this is my vocational perspective as someone who educates physics teachers. Of course, our main concern is the contribution of science, especially physics, to general education, which integrates many of the special topics mentioned. Philosophy of science comes in because it is not at all clear what science and physics is, and what of it should be taught, and how such chosen parts should be taught. I also take this opportunity to give an idea of the longstanding tradition of this discussion in Germany, connected with names like Wagenshein, Litt, Heisenberg and many others.  相似文献   

Amotivation and Indecision in the Decision-Making Processes Associated with University Entry     

Jae Yup Jung 《Research in higher education》2013,54(1):115-136

This study developed and tested two models that examined the decision-making processes of adolescents relating to entry into university, in terms of the extent to which they may be amotivated and undecided. The models incorporated variables derived from self-determination theory, expectancy-value theory, and research on occupational indecision. A modified version of a psychometrically rigorous survey instrument was used to collect data from 349 senior high school students attending three high schools in Sydney, Australia. Data were analysed using confirmatory factor analysis and structural equation modeling procedures. The refined versions of both models had good fit. The superior model suggested that: (a) family influences negatively predict amotivation with university entry (b) amotivation with university entry negatively predicts the valuing of interest/enjoyment and income due to university study, a desire for a “good” occupation, and expectations for success, and (c) amotivation with university entry positively predicts indecision with university entry.  相似文献   

Teachers’ roles in infants’ play and its changing nature in a dynamic group care context     

Jeesun Jung 《Early childhood research quarterly》2013

Using a qualitative research approach, this article explores teachers’ roles in infants’ play and its changing nature in an infant group care setting. Three infant teachers in a child care center were followed over three months. Observations, interviews, ongoing conversations, emails, and reflective notes were used as data sources. Findings revealed that the teachers took on various roles: observer, play follower/play partner, facilitator, commentator/interpreter, play supporter, play leader, play interrupter, safety/conflict manager, multiple-responder, and multiple-role taker. The nature of the teachers’ roles developed and changed over time in relation to the infants’ rapid growth, group dynamics, and infant–teacher relationships. This study suggests that infant teachers’ practice is complex, changing, and developmental as the group care context is dynamic and multilayered.  相似文献   

Wisdom from Korean Reentry Counseling Professionals: A Phenomenological Study of the Reentry Process     

Ae-Kyung Jung  Hang-Shim Lee  Alejandro Morales 《International journal for the advancement of counseling》2013,35(3):153-171

The purpose of this phenomenological study was to explore the common experience of Korean counseling professionals who returned to South Korea after obtaining their doctoral degrees in the United States. Individual semi-structured interviews were conducted with ten Korean counseling professionals to understand their cross-cultural experience. The data were analyzed by phenomenological methods of inquiry. Seven themes emerged from the analyses: Reminiscence, Differences and Comparisons, Benefits, Challenges, Coping, Application and Modification, and Meaning. The findings suggested how the setting and context in which the reentry process occurred contributed to the participants’ experience as well as the essence of the reentry process. Implications of the results are discussed in the light of cross-cultural counseling and for future research and practice.  相似文献   

Book review     

Mary E. Beadle  Jung‐Sook Lee 《广播与电子媒介杂志》2013,57(2):286-296

Kamalipour, Y. R., & Mowlana, H. (Eds.). (1994). Mass media in the Middle East: A comprehensive handbook. Westport, CN: Greenwood Press. 333 pages.

Boyd, D. (1993). Broadcasting in the Arab world: A survey of the electronic media in the Middle East (2nd ed.). Ames, IA: Iowa State University Press. 386 pages.

Hoyer, S., Lauk, E., & Vihalemm, P. (Eds.). (1993). Towards a civic society: The Baltic media's long road to freedom. Tartu, Estonia: Baltic Association for Media Research. 366 pages.

Silj, A. (Ed). (1992). The new television in Europe. London: John Libbey. 644 pages.

Noam, E., Komatsuzaki, S., & Conn, D. (Eds.). (1994). Telecommunications in the Pacific Basin. New York: Oxford University Press. 514 pages.

Englis, B. G. (Ed.). (1994). Global and multinational advertising. Hillsdale, NJ: Erlbaum. 266 pages.  相似文献